細(xì)胞名稱(chēng)

人肺腺癌細(xì)胞HCC78

貨物編碼

BFN60808857

產(chǎn)品規(guī)格

T25培養(yǎng)瓶x1

1.5ml凍存管x2

細(xì)胞數(shù)量

1x10^6

1x10^6

保存溫度

37℃

-198℃

運(yùn)輸方式

常溫保溫運(yùn)輸

干冰運(yùn)輸

安全等級(jí)

1

用途限制

僅供科研   2類(lèi)

培養(yǎng)體系

90%DMEM+10%FBS+1%三抗

培養(yǎng)溫度

37℃

二氧化碳濃度

5%

簡(jiǎn)介

人肺腺癌細(xì)胞HCC78取自65歲男性，該細(xì)胞源于DSMZ。

注釋

Part of: Cancer Cell Line Encyclopedia (CCLE) project.

Part of: COSMIC cell lines project.

Part of: MD Anderson Cell Lines Project.

Doubling time: ~60-70 hours (DSMZ).

Microsatellite instability: Stable (MSS) (Sanger).

Omics: Deep exome analysis.

Omics: Deep RNAseq analysis.

Omics: DNA methylation analysis.

Omics: Protein expression by reverse-phase protein arrays.

Omics: SNP array analysis.

Omics: Transcriptome analysis.

Miscellaneous: Direct author submission of STR profile from Girard L. and Gazdar A.F.

基因突變

SLC34A2-ROS1 gene fusion (PubMed=18083107; PubMed=25485619).

Homozygous for CDKN2A p.Arg58Ter (c.172C>T) (p.Pro72Leu; c.215C>T) (ClinVar=VCV000376310) (CCLE).

Homozygous for TP53 p.Ser241Phe (c.722C>T) (ClinVar=VCV000012359) (CCLE; Cosmic-CLP).

HLA信息

STR信息

Amelogenin        X,Y

CSF1PO        11,12

D1S1656        15.3,17.3

D2S441        11,14

D2S1338        17,21

D3S1358        16,17

D5S818        11,12

D7S820        9

D8S1179        13,14

D10S1248        14,16

D12S391        19,21

D13S317        8,12

D16S539        11,13

D18S51        12,16

D19S433        16,16.2

D21S11        29,30

D22S1045        15,16

DYS391        12

FGA        21,22

Penta D        7,11

Penta E        11,12

TH01        7,9.3

TPOX        8,11

vWA        15,18

參考文獻(xiàn)

PubMed=28196595; DOI=10.1016/j.ccell.2017.01.005

Li J., Zhao W., Akbani R., Liu W., Ju Z., Ling S., Vellano C.P., Roebuck P., Yu Q., Eterovic A.K., Byers L.A., Davies M.A., Deng W., Gopal Y.N.V., Chen G., von Euw E.M., Slamon D.J., Conklin D., Heymach J.V., Gazdar A.F., Minna J.D., Myers J.N., Lu Y., Mills G.B., Liang H.

Characterization of human cancer cell lines by reverse-phase protein arrays.

Cancer Cell 31:225-239(2017)

PubMed=30038707; DOI=10.18632/oncotarget.25642

Du L., Zhao Z., Suraokar M., Shelton S.S., Ma X., Hsiao T.-H., Minna J.D., Wistuba I., Pertsemlidis A.

LMO1 functions as an oncogene by regulating TTK expression and correlates with neuroendocrine differentiation of lung cancer.

Oncotarget 9:29601-29618(2018)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747

Dutil J., Chen Z., Monteiro A.N., Teer J.K., Eschrich S.A.

An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.

Cancer Res. 79:1263-1273(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3

Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., McDonald E.R. III, Barretina J., Gelfand E.T., Bielski C.M., Li H., Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., Lu Y., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., Sellers W.R.

Next-generation characterization of the Cancer Cell Line Encyclopedia.

Nature 569:503-508(2019)