細胞名稱

非洲綠猴腎細胞Vero E6

貨物編碼

BFN60803966

產品規(guī)格

T25培養(yǎng)瓶x1

1.5ml凍存管x2

細胞數量

1x10^6

1x10^6

保存溫度

37℃

-198℃

運輸方式

常溫保溫運輸

干冰運輸

安全等級

1

用途限制

僅供科研           1類

培養(yǎng)體系

DMEM高糖培養(yǎng)基（Hyclone）+10%胎牛血清（Gibco）+1%雙抗（Hyclone）

培養(yǎng)溫度

37℃

二氧化碳濃度

5%

簡介

非洲綠猴腎細胞Vero E6細胞是VERO76細胞的一個克隆，1979年由P.J.Price通過有限稀釋法獲得。 

注釋

Group: Non-human primate cell line.

Group: Vaccine production cell line.

Part of: Naval Biosciences Laboratory (NBL) collection (transferred to ATCC in 1982).

Biotechnology: Used for the production of the Japanese encephalitis virus (JEV) vaccine (Trade name: IXIARO/JESPECT).

Biotechnology: Used for the production of the inactivated poliovirus vaccine (Trade name: Imovax Polio).

Biotechnology: Used for the production of the Purified Vero-cell Rabies Vaccine (PVRV) (Trade name: VERORAB).

Biotechnology: Used for the production of the Chromatographically Purified Rabies Vaccine (CPRV).

Biotechnology: Used for the production of the live oral rotavirus vaccine (Trade Name: Rotarix).

Biotechnology: Used for the production of the tetravalent dengue vaccine (Trade Name: Dengvaxia).

Characteristics: Susceptible to SARS coronavirus (SARS-CoV) infection (PubMed=16494729).

Characteristics: Susceptible to SARS coronavirus 2 (SARS-CoV-2) infection . But Vero C1008 (often known as Vero E6) (CVCL_0574) seems to be more appropriate for amplification and quantification (DOI=10.1101/2020.03.02.972935).

Omics: Genome sequenced.

Anecdotal: Verda reno means green kidney in Esperanto and Vero means truth also in Esperanto.

Derived from sampling site: Kidney; epithelium.

STR信息

/

參考文獻

PubMed=24975811; DOI=10.1016/j.vaccine.2014.06.045

Donis R.O., Chen I.-M., Davis C.T., Foust A., Hossain M.J., Johnson A., Klimov A., Loughlin R., Xu X., Tsai T., Blayer S., Trusheim H., Colegate T., Fox J., Taylor B., Hussain A., Barr I., Baas C., Louwerens J., Geuns E., Lee M.-S., Venhuizen O., Neumeier E., Ziegler T.

Performance characteristics of qualified cell lines for isolation and propagation of influenza viruses for vaccine manufacturing.

Vaccine 32:6583-6590(2014)

PubMed=25267831; DOI=10.1093/dnares/dsu029

Osada N., Kohara A., Yamaji T., Hirayama N., Kasai F., Sekizuka T., Kuroda M., Hanada K.

The genome landscape of the African green monkey kidney-derived Vero cell line.

DNA Res. 21:673-683(2014)

PubMed=25903999; DOI=10.1002/biot.201400388

Genzel Y.

Designing cell lines for viral vaccine production: where do we stand?

Biotechnol. J. 10:728-740(2015)

DOI=10.1101/2020.03.02.972935

Harcourt J., Tamin A., Lu X., Kamili S., Sakthivel S.K., Murray J., Queen K., Tao Y., Paden C.R., Zhang J., Li Y., Uehara A., Wang H., Goldsmith C., Bullock H.A., Wang L., Whitaker B., Lynch B., Gautam R., Schindewolf C., Lokugamage K.G., Scharton D., Plante J.A., Mirchandani D., Widen S.G., Narayanan K., Makino S., Ksiazek T.G., Plante K.S., Weaver S.C., Lindstrom S., Tong S., Menachery V.D., Thornburg N.J.

Isolation and characterization of SARS-CoV-2 from the first US  patient.

bioRxiv 2020:972935-972935(2020)